Review



thtpa antibody (3f6)  (Bio-Techne corporation)


Bioz Verified Symbol Bio-Techne corporation is a verified supplier
Bioz Manufacturer Symbol Bio-Techne corporation manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Bio-Techne corporation thtpa antibody (3f6)
    Thtpa Antibody (3f6), supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thtpa antibody (3f6)/product/Bio-Techne corporation
    Average 90 stars, based on 2 article reviews
    thtpa antibody (3f6) - by Bioz Stars, 2026-02
    90/100 stars

    Images



    Similar Products

    thtpa antibody (3f6)  (Bio-Techne corporation)
    90
    Bio-Techne corporation thtpa antibody (3f6)
    Thtpa Antibody (3f6), supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thtpa antibody (3f6)/product/Bio-Techne corporation
    Average 90 stars, based on 1 article reviews
    thtpa antibody (3f6) - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier
    anti thtpa antibodies  (Proteintech)
    93
    Proteintech anti thtpa antibodies
    Both cell lines were analyzed under six conditions: control for 24 hours (C-24); incubation in thiaminase for 24 hours (T-24); control for 48 hours (C-48); thiaminase for 48 hours (T-48); rapamycin for 48 hours (R-48); and both rapamycin and thiaminase for 48 hours (R+T-48). The median is indicated by the bar in the center of the rectangle, the rectangle dimensions reflect the range of the two mid-quartile values, and the outer bars represent the ranges of all of the values. The data represent four independent experiments. For C-48 vs T-48 and C-48 vs T+R-48 comparisons, ** indicates p<0.05 and * indicates 0.05<p<0.1). A. Internal validation in RS4 cells, showing the expected decrease in thiamine and thiamine diphosphate in thiaminase treated cells, an increase in thiazole, the product of thiaminase cleavage of thiamine in the thiaminase-treated cells and the appearance of rapamycin only in the rapamycin treated cells. B. Validation of thiaminase-mediated inhibition of transketolase by demonstration of the accumulation of substrate surrogates ribose and ribulose in both RS4 and MCF-7 cell lines at 48 hours. C. Immunoblot analysis of thiamine pyrophosphate <t>kinase</t> <t>(TPK1)</t> and thiamine triphosphatase <t>(THTPA)</t> in RS4 and MCF-7 cells treated with thiaminase, rapamycin or both. D. Immunoblot analysis of pyruvate kinase isozymes M1 and M2 and carnitine palmitoyl transferase (CPT1) in RS4 and MCF-7 cells treated with thiaminase, rapamycin or both.
    Anti Thtpa Antibodies, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti thtpa antibodies/product/Proteintech
    Average 93 stars, based on 1 article reviews
    anti thtpa antibodies - by Bioz Stars, 2026-02
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Both cell lines were analyzed under six conditions: control for 24 hours (C-24); incubation in thiaminase for 24 hours (T-24); control for 48 hours (C-48); thiaminase for 48 hours (T-48); rapamycin for 48 hours (R-48); and both rapamycin and thiaminase for 48 hours (R+T-48). The median is indicated by the bar in the center of the rectangle, the rectangle dimensions reflect the range of the two mid-quartile values, and the outer bars represent the ranges of all of the values. The data represent four independent experiments. For C-48 vs T-48 and C-48 vs T+R-48 comparisons, ** indicates p<0.05 and * indicates 0.05<p<0.1). A. Internal validation in RS4 cells, showing the expected decrease in thiamine and thiamine diphosphate in thiaminase treated cells, an increase in thiazole, the product of thiaminase cleavage of thiamine in the thiaminase-treated cells and the appearance of rapamycin only in the rapamycin treated cells. B. Validation of thiaminase-mediated inhibition of transketolase by demonstration of the accumulation of substrate surrogates ribose and ribulose in both RS4 and MCF-7 cell lines at 48 hours. C. Immunoblot analysis of thiamine pyrophosphate kinase (TPK1) and thiamine triphosphatase (THTPA) in RS4 and MCF-7 cells treated with thiaminase, rapamycin or both. D. Immunoblot analysis of pyruvate kinase isozymes M1 and M2 and carnitine palmitoyl transferase (CPT1) in RS4 and MCF-7 cells treated with thiaminase, rapamycin or both.

    Journal: PLoS ONE

    Article Title: Metabolic Effects of Acute Thiamine Depletion Are Reversed by Rapamycin in Breast and Leukemia Cells

    doi: 10.1371/journal.pone.0085702

    Figure Lengend Snippet: Both cell lines were analyzed under six conditions: control for 24 hours (C-24); incubation in thiaminase for 24 hours (T-24); control for 48 hours (C-48); thiaminase for 48 hours (T-48); rapamycin for 48 hours (R-48); and both rapamycin and thiaminase for 48 hours (R+T-48). The median is indicated by the bar in the center of the rectangle, the rectangle dimensions reflect the range of the two mid-quartile values, and the outer bars represent the ranges of all of the values. The data represent four independent experiments. For C-48 vs T-48 and C-48 vs T+R-48 comparisons, ** indicates p<0.05 and * indicates 0.05

    Article Snippet: Anti- PKM1; anti-BCAT2; anti-TPK1 and anti-THTPA antibodies were purchased from Proteintech Group, Inc. (Chicago, IL).

    Techniques: Control, Incubation, Biomarker Discovery, Inhibition, Western Blot